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Image Search Results
Journal: FEMS microbiology letters
Article Title: Free-living freshwater amoebae differ in their susceptibility to the pathogenic bacterium Legionella pneumophila.
doi: 10.1111/j.1574-6968.2008.01387.x
Figure Lengend Snippet: Fig. 1. Effect of Legionella pneumophila on the growth of different amoebae in coculture. (a) Effect of the bacterial infection on the growth of amoebae. The different amoebae were cultured at 37 1C for up to 4 days (d3 and d4 represent, respectively, day 3 and day 4 in culture) either with (grey bars) or without (black bars) L. pneumophila Paris at an MOI of 50. White bars represent the number of amoebae on day 0 of experiments. The data are expressed as the number of amoebae mL1 of medium and are the mean SEM of four to eight independent experiments. Statistical differences (Student’s t-test) between the growth of amoebae cultured either with or without bacteria are indicated (P o 0.01; P o 0.001). (b and c) Effect of L. pneumophila on monolayer formation by amoebae. Representative phase-contrast images of the different amoebae cultured in 24-well plates either with or without the strains of L. pneumophila at an MOI of 50 for 3 days (b) or 7 days (c). Note the destruction of monolayers of Hartmannella and Acanthamoeba cultured in the presence of the bacteria when compared with controls and also the relative resistance of Willaertia magna (strain c2c). Also, note the destruction of monolayers of W. magna Z503 cultured in the presence of the bacteria for 7 days when compared with controls and the c2c strain.
Article Snippet: The amoebae used in this study were A. castellanii (By 02.2.4) and H. vermiformis (Ax.5.2e4b), two species known to support L. pneumophila and two strains of Willaertia genus, hitherto untested: W. magna, Z 503 (ATCC 50035) and
Techniques: Infection, Cell Culture, Bacteria
Journal: FEMS microbiology letters
Article Title: Free-living freshwater amoebae differ in their susceptibility to the pathogenic bacterium Legionella pneumophila.
doi: 10.1111/j.1574-6968.2008.01387.x
Figure Lengend Snippet: Fig. 3. Ultrastructure of Acanthamoeba castellanii and Willaertia magna infected by Legionella pneumophila after 36 h in coculture. (a, d and g) Different stages of A. castellanii infection by the L. pneumophila Paris. Micrographs (b), (e) and (h) show the few cells (see Table 1 for details) of W. magna c2c that were found to be infected by L. pneumophila Paris. (c, f and i) Typical W. magna Z503 infected by L. pneumophila Lens. (a) First stage of A. castellanii infection: the bacteria are contained in phagosomes surrounded by a rough endoplasmic reticulum (rer). (b) Single L. pneumophila not surrounded by rer in W. magna c2c. (c) Willaertia magna Z503 displaying a clearly delimited replicative phagosome filled with L. pneumophila Lens. (d) Massively infected prelytic A. castellanii displaying a contractile vacuole (CV). N, nucleus. (e) One of the two W. magna c2c (2/133 cells observed) displaying a replicative phagosome. (f) Massively infected prelytic W. magna Z503. (g–i) Different morphological aspects of L. pneumophila Paris internalized within Acanthamoeba (g) and Willaertia c2c (h) and of L. pneumophila Lens within Willaertia Z503 (i). Bacteria are indicated by arrows on (b), (c), (f), (g), (h) and (i).
Article Snippet: The amoebae used in this study were A. castellanii (By 02.2.4) and H. vermiformis (Ax.5.2e4b), two species known to support L. pneumophila and two strains of Willaertia genus, hitherto untested: W. magna, Z 503 (ATCC 50035) and
Techniques: Infection, Bacteria